Table 2 Origin of the samples included in the study (n=44) and the respective results. Copy numbers were then normalized to DNA concentration and expressed as per 100 ng of genomic DNA
Sample No. | Origin | Genotype | ELISA | qPCR result (no. of BLV DNA copies per 100 ng of total DNA)a | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Â | Â | Â | Â | qPCR1 (pol) | qPCR2 (pol) | qPCR3 (LTR) | qPCR4 (pol) | qPCR5 (pol) | ddPCR6 (env) | qPCR7d (pol) | qPCR8d (pol) | qPCR9d (pol) | qPCR10d (pol) | qPCR11d (pol) |
1 | Poland | G4 | + | 17253.9 | 7820.2 | 962541.0 | 285583.3 | 3490.2 | 4480.0 | 36700.0 | 10820.0 | 24700.0 | 11613.8 | 84463.7 |
2 | Poland | G4 | + | 2042.6 | 465.6 | 48555.9 | 19950.0 | 947.4 | 442.0 | 159.0 | 489.0 | 209.0 | 835.8 | 20127.2 |
3 | Ukraine | G4 | + | 30365.9 | 8329.2 | 184258.8 | 398733.3 | 19476.0 | 8320.0 | 50800.0 | 28699.0 | 16400.0 | 13441.1 | 165171.6 |
4 | Ukraine | G8 | + | 6994.0 | 4818.0 | 14457.6 | 120130.0 | 7180.4 | 4.4 | 5710.0 | 5459.0 | 3900.0 | 3384.3 | 52440.6 |
5 | Ukraine | G8 | + | 6653.5 | 6354.8 | 64721.9 | 109896.7 | 4753.6 | 1734.0 | 7110.0 | 9199.0 | 1660.0 | 46.3 | 249756.1 |
6 | Ukraine | bNs | + | 11586.5 | 10479.8 | 191854.7 | 217700.0 | 11272.9 | 1501.2 | 4970.0 | 12250.0 | 15500.0 | 9476.4 | 48900.5 |
7 | Ukraine | Ns | + | 89.1 | 52.3 | 43.3 | 5613.3 | 7.7 | 10.4 | 12.6 | 51.0 | 1.7 | 27.0 | 502.7 |
8 | Poland | G8 | + | 1146.9 | 381.9 | 119.5 | 8993.3 | 479.7 | NEG | 173.0 | 267.0 | 118.0 | 388.9 | 2738.9 |
9 | Poland | G7 | + | 0.3 | NEG | NEG | NEG | 1.0 | NEG | 12.3 | 17.0 | NEG | NEG | 6.1 |
10 | Moldova | G7 | + | 130.5 | 1345.1 | 2533.8 | 7090.0 | 21.1 | 76.0 | 188.0 | 224.0 | 3.4 | 207.3 | 2935.0 |
11 | Ukraine | Ns | + | 1618.1 | 1274.6 | 308.8 | 18996.7 | 934.5 | NEG | 1480.0 | 948.0 | 209.0 | 816.1 | 8483.0 |
12 | Moldova | G7 | + | 5964.5 | 6473.7 | 127613.4 | 235810.0 | 1673.2 | 5320.0 | 21600.0 | 11541.0 | 5950.0 | 16235.6 | 171017.9 |
13 | Poland | G4 | + | 41743.1 | 2816.6 | 580303.9 | 378866.7 | 15555.0 | 6100.0 | 21300.0 | 16300.0 | 18400.0 | 28410.3 | 505675.6 |
14 | Poland | Ns | + | 2.0 | NEG | NEG | NEG | 2.5 | NEG | 11.0 | 4.0 | 0.1 | 2.1 | 97.9 |
15 | Poland | G8 | + | 13.0 | NEG | NEG | NEG | 6.4 | NEG | NEG | 12.0 | 0.1 | 3.9 | 170.7 |
16 | Moldova | Ns | + | 6.8 | NEG | 14.1 | NEG | 4.0 | 4.6 | 40.8 | 6.0 | 0.1 | 1.8 | 97.8 |
17 | Poland | G4 | + | 40.2 | 69.6 | 1002.7 | 736.7 | 11.3 | 12.0 | 18.8 | 18.0 | 1.2 | 19.8 | 368.3 |
18 | Poland | G7 | + | 1.5 | NEG | 306.5 | 536.7 | NEG | 3.8 | 23.7 | NEG | 0.1 | 2.5 | 35.0 |
19 | Poland | G7 | + | 18092.8 | 807.5 | 246292.1 | 432900.0 | 3215.4 | 7760.0 | 47700.0 | 16849.0 | 19800.0 | 18718.6 | 218610.3 |
20 | Moldova | G7 | + | 2188.4 | 19195.8 | 268180.6 | 360833.3 | 2957.0 | 5700.0 | 29100.0 | 3863.0 | 985.0 | 19494.0 | 129483.2 |
21 | Poland | G4 | + | 423.9 | 1593.3 | 295.7 | 8503.3 | 202.7 | 214.0 | 460.0 | 267.0 | 7.4 | 277.0 | 5211.4 |
22 | Poland | G4 | + | 33005.6 | 14517.9 | 335322.0 | 364700.0 | 13565.7 | 12040.0 | 15400.0 | 4539.0 | 12200.0 | 28424.2 | 152574.9 |
23 | Poland | G4 | + | 4776.8 | 4750.2 | 197.2 | 80110.0 | 3385.1 | 1800.0 | 13200.0 | 2050.0 | 1280.0 | 2269.0 | 71669.1 |
24 | Poland | G7 | + | 4.9 | NEG | 81.6 | NEG | 1.9 | 8.2 | 34.1 | 5.0 | 0.1 | 1.4 | 99.2 |
25 | Poland | G8 | + | 38.1 | 1152.2 | 8.0 | 533.3 | 30.0 | NEG | 144.0 | 26.0 | 0.2 | 33.8 | 524.7 |
26 | Poland | G8 | + | 1017.4 | 921.7 | 233.9 | 19566.7 | 828.3 | NEG | 1850.0 | 850.0 | 74.4 | 781.9 | 9819.5 |
27 | Poland | G4 | + | 41388.9 | 9513.7 | NEG | 218583.3 | 10410.8 | 4000.0 | 30800.0 | 6033.0 | 10900.0 | 18878.0 | 131461.4 |
28 | Poland | G7 | + | 1.0 | NEG | 480.1 | NEG | NEG | NEG | 17.5 | 9.0 | 0.3 | 0.7 | NEG |
29 | Poland | G4 | + | 23913.0 | 1886.5 | 173311.8 | 334533.3 | 16739.7 | 7570.0 | 12700.0 | 2476.0 | 7590.0 | 27163.2 | 170384.4 |
30 | Poland | G8 | + | 7.8 | 113.5 | NEG | 326.7 | 4.8 | NEG | 6.3 | 12.0 | 1.0 | 3.8 | 41.5 |
31 | Pakistan | G6 | + | 8920.5 | 3293.8 | 13751.5 | 57813.3 | 1633.2 | 860.0 | 3570.0 | 2386.0 | 825.0 | 3197.8 | 49053.1 |
32 | Poland | cNa | - | aNEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG |
33 | Pakistan | G6 | + | 1308.8 | 417.5 | 29221.6 | 15846.7 | 387.0 | 351.8 | 1490.0 | 800.0 | 103.0 | 856.7 | 18479.7 |
34 | Pakistan | G1 | + | 1.6 | NEG | 259.8 | NEG | NEG | NEG | NEG | 8.0 | 0.1 | 0.7 | NEG |
35 | Pakistan | G1 | + | 7000.0 | 1082.7 | 104381.2 | 76063.3 | 5485.9 | 1700.0 | 9480.0 | 4156.0 | 1200.0 | 3869.7 | 23367.2 |
36 | Pakistan | G6 | + | 326.3 | 52.8 | 2742.6 | 5933.3 | 240.3 | 176.0 | 187.0 | 209.0 | 16.2 | 188.2 | 1499.4 |
37 | Canada | G1 | + | 385.7 | 3083.5 | 437.2 | 9630.0 | 245.0 | 132.0 | 430.0 | 326.0 | 26.5 | 169.9 | 4185.5 |
38 | Canada | G1 | + | 1452.8 | 84.4 | 78032.9 | 24483.3 | 911.6 | 338.0 | 2000.0 | 949.0 | 133.0 | 591.4 | 16415.2 |
39 | Canada | G1 | + | 1245.8 | 871.8 | 6963.9 | 19863.3 | 667.6 | 268.0 | 2140.0 | 989.0 | 111.0 | 410.5 | 15095.0 |
40 | USA | G1 | + | 30652.2 | 4620.7 | 406854.9 | 269223.3 | 10184.5 | 4040.0 | 25900.0 | 29479.0 | 2660.0 | 9719.0 | 70397.3 |
41 | USA | G1 | + | 51885.2 | 23907.4 | 607640.5 | 179676.7 | 22906.4 | 5280.0 | 32400.0 | 51006.0 | 6980.0 | 16097.0 | 458976.1 |
42 | Belgium | G4 | pBLV344e | 4.07E+10 | 4.15E+09 | 4E+09 | 7E+06 | 1.45E+09 | 2.9E+07 | 7.48E+09 | 2.27E+08 | 2.55E+11 | 2.59E+09 | 1.22E+11 |
43 | USA | G1 | BLV-FLKf | 307692.3 | 162180.6 | 863440.0 | 3250666.7 | 75773.5 | 28480.0 | 168000.0 | 244769.0 | 482000.0 | 138382.8 | 4286730.3 |
44 | Poland | G8 | + | 1.7 | 41.8 | NEG | 763.3 | 10.4 | NEG | NEG | 5.0 | 0.2 | 2.0 | 5.9 |
Total | POSITIVE | 43 | 43 | 35 | 37 | 36 | 40 | 32 | 40 | 42 | 42 | 42 | 41 | |
NEGATIVE | 1 | 1 | 9 | 7 | 8 | 4 | 12 | 4 | 2 | 2 | 2 | 3 | ||