Inter-laboratory comparison of eleven quantitative or digital PCR assays for detection of proviral bovine leukemia virus in blood samples

Table 3 Assessment of qPCR results by pairwise comparison

PCR assay	Kappa value or agreement^a with the results of the following PCR assay
	qPCR1	qPCR2	qPCR3	qPCR4	qPCR5	ddPCR6	qPCR7	qPCR8	qPCR9	qPCR10	qPCR11
qPCR1	-	0.166	0.219	0.189	0.377	0.117	0.377	0.656	0.656	0.656	0.482
qPCR2	81.8%	-	0.391	0.927	0.560	0.441	0.384	0.313	0.313	0.312	0.443
qPCR3	86.4%	81.8%	-	0.438	0,075	0.539	0.486	0.163	0.402	0.402	0.115
qPCR4	84.1%	97.7%	84.1%	-	0.431	0.488	0.431	0.137	0.353	0.352	0.495
qPCR5	93.2%	88.6%	79.5%	86.4%	-	0.276	0.450	0.645	0.290	0.290	0.845
ddPCR6	75.0%	79.6%	84.1%	81.8%	77.3%	-	0.421	0.070	0.225	0.225	0.326
qPCR7	93.2%	84.1%	88.6%	86.4%	90.9%	81.8%	-	0.290	0.290	0.290	0.535
qPCR8	97.7%	84.1%	84.1%	66.8%	95.5%	72.7%	90.9%	-	0.476	0.476	0.365
qPCR9	97.7%	84.1%	88.6%	86.4%	90.9%	77.3%	90.9%	95.5%	-	1.00	0.365
qPCR10	97.7%	84.1%	88.6%	86.4%	90.9%	77.3%	90.9%	95.5%	100%	-	0.365
qPCR11	95.5%	86.4%	81.8%	88.6%	97.7%	79.6%	93.2%	93.2%	93.2%	93.2%	-

^aValues above the blank cells are kappa values; values below the blank cells are percentages of agreement

ISSN: 1746-6148